Yes they make a big difference.
The two ring gets thinner/richer faster giving a richer mix 5/16 to 3/4 throttle.
It works best to feed thirsty pipes/porting.
The one ring's med-range gets super lean when the pipe starts flowing/hitting giving you crazy death rattle and ping from hell.
And this will over heat/seize your kit, if your lucky it will just burn a vent in the top of your piston to cool things down.
I like using the #24 slide with mod's, 2.17 or the richer 2.19 Atomizer, two ring needle, on 14mm & 15mm bing carbs.
On bikes with kit's and or porting with good pipes.